Evolving high-throughput approaches to assess the environmental risk of sewage water at molecular level: comprehensive study.

Dissolved organic matter (DOM) especially anthropogenic compounds in sewage systems affects their ultimate fate in the environment which is challenging to ascertain the heterogenic nature of the compound and causes co-occurring effects in most aquatic samples. So, our study have focused on current approaches to the chemical and structural characterization of DOM with the detailed classification of individual compounds such as the molecular levels of volatile organic, inorganic materials, drugs and endocrine disrupting compounds. Analytical techniques for example high performance gas chromatography-mass spectrometry (GCMS) with high-resolution liquid chromatography (HR-LCMS), X-ray diffraction (XRD) and three-dimensional fluorescence excitation emission matrix (3D-EEM) has resulted in advancing the parametric studies. In addition, the toxicological assessment of an aquatic organism (zebrafish as a model) has ensued in enlightening the risk of contaminated sources. The result of the research highlighted the efficacy of high-throughput approaches to assess the environmental impact of sewage water.

An overview of organic matters in municipal wastewater: Removal via self-assembly flocculating mechanism and the molecular level characterization.

On considering the critical issues in attaining stringent water quality standards and not creating any environmental impacts, we focused for the first time the economically feasible, emerging technology known as Self-assembly flocculating (Saf process). In which, the study investigated the applicability of bioflocculant (a biopolymer-self-assembly in nature) act as a surrogates on relying the removal of broad spectrum of substances under optimized conditions (Dosage: 90 mg/L; pH: 7; CaCl2). On using different techniques, the results have proved in removing the organic matter such as pharmaceuticals (Gentamycin, Cholecalciferol, Fluvoxamine, 3-OH Desogestrel, and Pheniramine), endocrine disturbing compounds [Phthalic acid, Benzene, 1, 2, 4 -Trimethoxy-5-(1-Propenyl)-, Benzene, 1, 2-Dimethoxy-4-(2-Propenyl)-, 1, 2-Benzenedicarboxylic Acid, 3-Cyclohexen-1-ol], fluorescent components (Polysaccharide like material), and others. The toxicological assessment of self-assembly bioflocculant implemented on zebra fish were statistically correlated [r = 0.95, p < 0.01 and 0.05 for P1WW; r = 0.91, p < 0.01 and 0.05 for P2WW] and [r = 0.7 5, p < 0.05 for P1WT; r = 0.095, p < 0.01 and 0.05 for P2WT]. This integrated approach supplemented further information of zeta potential (-16 mV in P1WW and -14.6 mV in P2WW decreased to -1.05 mV and -1.56 mV) with particle size distribution to explain via Saf process. In this research, the new insight has established non-toxic, self-assembly, biodegradable, bioflocculant for effective bioremediation.

Characterization of self-assembled bioflocculant from the microbial consortium and its applications.

Bioflocculant has been recently exploited in various research activities. In this research, we report for the first time that a novel bioflocculant can self-assemble into nanoparticles with an irregular structure in solution. Bioflocculant has been developed from novel consortium encompassing Enterococcus faecalis, Proteus mirabilis, Lysini bacillus sp., inoculated into the modified medium such as hydrolyzed wheat bran and rice bran extract. Characterization of bioflocculant shows that it is made up of mannose, fructose, raffinose, and galactose with a slightly negative charges. They are further characterized by FTIR and XPS. 3D-EEM and MALDITOF-MS are confirmed the proteoglycan nature of the bioflocculant. These bioflocculant not only exhibits greater biosorption of heavy metals by self-flocculating (or) self-aggregating activity, but also possesses mosquitocidal and anti-biofilm activity due to its cell surface modification. Further research have to be carried out regarding the mechanism of self-assembly nature of bioflocculant into nanoparticle in solution which provide a new path for bioremediation and biomedical application.